Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using RM159 at 0.1 ug/mL, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells .
Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green).