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ELISA of mouse immunoglobulins shows RMG05
reacts to the Fab region of mouse IgG1, IgG2a,
IgG2b, and IgG3; no cross reactivity with IgM, IgA,
IgE, human IgG, rat IgG, and rabbit IgG. The plate
was coated with 50 ng/well of different immunoglo-
bulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of
RMG05 was used as the primary antibody. An
alkaline phosphatase conjugated anti-goat IgG as
the secondary antibody.