RM155 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non- modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM155 at 2.0 ug/mL, showed a band of histone H3 trimethylated at Lysine 36 (K36me3) in HeLa cells.
ChIP performed on HeLa cells using H3K36me3 antibody (RM155, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
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