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Anti-Complement component C9 (human) ABS 004-53
品牌：
貨號：ABS 004-53
規格：200 μg
貨期：

Anti-Complement component C9 (human)
商品詳情 參考文獻 相關資料
Cat. No.
ABS 004-53
Product name
Anti-Complement component C9 (human)
Description

Mouse monoclonal antibody

Specificity

ABS 004-53 is specific for complement factor C9

Immunogen

Complement factor C9

Application
ELISA
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
735
Clone number
C9 53
Epitope

Not determined

Content

Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8oC without exposure to light. No precautions necessary during handling.
Target

Complement factor C9 is a 71kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 μg/mL, and the level is up regulated from hepatocytes by IFN-g.?

Application 1

ELISA

ABS 004-53 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.
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